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Theory to Practice Assignment-3

Theory to Practice Assignment-3

Q 1. Know the components of a nucleotide. 2. Know how the backbone of DNA (handrails of the double helix) is formed. 3. Know how the nitrogen bases base pair with each other according to the complementary base pairing rules and which bonds (hydrogen bonds) are responsible for binding the nitrogen bases of two strands with each other. 4. You should be knowing how DNA replicates. 5. You should know the name of the main enzyme involved in DNA replication. 6. What is gene cloning and what is the application of gene cloning. 7. Know the steps of gene cloning. 8. Know the terms- transgenic organism, restriction enzymes, plasmids, recombinant DNA, transformation. 9. Know the term somatic cell nuclear transfer (SCNT). 10. Know the steps how the sheep DOLLY was cloned. 11. What is DNA typing or DNA Fingerprinting. 12. What is PCR (Polymerase Chain Reaction) and what is the application of PCR. 13. What are Short Tandem Repeats (STR).

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The laboratory activity this week defines how the DNA extraction is needed to fully process the acquisition of hereditary and genetic information of an individual. The DNA molecules have something to do with the things around us, and I believe that each and every living organisms contains DNA structures relevant mto studying their speci further. For instance, DNA can help scientists in discovering the biologic relationship of one person to another; might as well as the DNA molecules plays a pivot role in the field of forensics wherein crime scene investigators collect fingerprints or saliva or bloods and can be used by forensic scientists in identifying who are in the crime scene at those times.