Q 1. Know the components of a nucleotide. 2. Know how the backbone of DNA (handrails of the double helix) is formed. 3. Know how the nitrogen bases base pair with each other according to the complementary base pairing rules and which bonds (hydrogen bonds) are responsible for binding the nitrogen bases of two strands with each other. 4. You should be knowing how DNA replicates. 5. You should know the name of the main enzyme involved in DNA replication. 6. What is gene cloning and what is the application of gene cloning. 7. Know the steps of gene cloning. 8. Know the terms- transgenic organism, restriction enzymes, plasmids, recombinant DNA, transformation. 9. Know the term somatic cell nuclear transfer (SCNT). 10. Know the steps how the sheep DOLLY was cloned. 11. What is DNA typing or DNA Fingerprinting. 12. What is PCR (Polymerase Chain Reaction) and what is the application of PCR. 13. What are Short Tandem Repeats (STR).